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Signagen/AAV-CMV-FLPo (AAV Serotype 6)/SL101458/>10 µL (Trial Pack)
ProductCategory: Pre-maderecombinantAAV;AAV-CMV-FLPo;AAV-FLPo;AAV6-CMV-FLPo;AAV6-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheφintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV6-CMV-FLPopre-packagedrAAVinSEROtype6(withcapsidfromAAVserotype6and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderCMVpromoter. Readytouseformat.
Titer: >1x10^13 VG/mL
Size: > 30 µL(StdPack)
StorageBuffer: SterilePBS
InfectionProtocol: 
VectorMap: 
